As shown in Figure 13, the reaction rate increases with temperature to a maximum level, then abruptly declines with further increase of temperature.Because most animal enzymes rapidly become denatured at temperatures above 40°C, most enzyme determinations are carried out somewhat below that temperature.Catalase is one of the most recognized enzymes found in living organisms.
Factors include: concentration of the enzyme, temperature, p H level, concentration of the substrate, and inhibitors.
This lab shows the affects these factors have on the rate of reaction between catalase, an enzyme found in potatoes, and hydrogen peroxide, the substrate.
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Like most chemical reactions, the rate of an enzyme-catalyzed reaction increases as the temperature is raised.
A ten degree Centigrade rise in temperature will increase the activity of most enzymes by 50 to 100%.
Catalase breaks down hydrogen peroxide, a very harmful oxidizing agent for cells (Catalase).
A single catalase molecule can break down millions of hydrogen peroxide molecules in a given moment.
Catalase breaks down hydrogen peroxide into water and oxygen.
Hydrogen peroxide is a natural waste product which forms when the body breaks down fatty acids and converts that into energy.