Uwo Thesis Binding

Tags: Problem Solving Techniques For StudentsWrite Essay PostmodernismVoorbeeld Titelblad EssayPay For Essays OnlineChild Development Research PaperRutgers College Essay HelpVoip Phd ThesisWriting A Good Thesis Statement For An Analytical EssayCollege DissertationSteroids Persuasive Essay

Isd B contains two NEAT (NEAr Iron Transporter) domains, and both domains and the intervening linker are needed for efficient heme uptake from Hb. I created the figures and wrote the first draft of relevant parts of the methods and results sections pertaining to my work. Lindsay Eltis assisted with the analysis of the heme transfer kinetics. Approval was provided by the UBC Clinical Research Ethics Board, Certificate Number H11- 03395, under Project Title “Hemoglobin binding by the Isd B receptor of Staphylococcus aureus.

In the first part of my study, the molecular mechanism of heme transfer from Hb to Isd B was investigated using site-directed mutagenesis. This project required Biohazard approval for the handling of Staphylococcus aureus and Escherichia coli. (2010) Hemoglobin depletion from plasma: Considerations for proteomic discovery in Sickle Cell disease and other hemolytic processes.

To date, the Iron Surface Determinant (Isd) system is the only heme utilization pathway identified in S. Isd B is the primary receptor at the cell surface that binds Hb to extract heme. Haptoglobin blocks formation of a key intermediate in heme transfer from hemoglobin to Isd B of Staphylococcus aureus. Angelé Arrieta cloned the YFYF variant expression plasmid.

Heme is then transferred to Isd A or Isd C, which then relay it to other Isd proteins for internalization. I produced and purified YFYF protein and carried out the activity assays in collaboration with Catherine. aureus growth experiments on Hb as a sole iron source and demonstrating inhibition by Hp. iv This project required Ethics Approval for purifying hemoglobin from fresh human blood.

Heme is then transferred to Isd A or Isd C, which then relay it to other Isd proteins for internalization. (1985) Uptake of iron from hemoglobin and the haptoglobin-hemoglobin complex by hemolytic bacteria.

Isd B contains two NEAT (NEAr Iron Transporter) domains, and both domains and the intervening linker are needed for efficient heme uptake from Hb.

[email protected] theses will have priority in many search engines.

Theses will be uploaded to the University of Western Ontario Library catalogue at regular intervals.

Residues in the heme-binding pocket were identified by inspection of a crystal structure of the complex of Isd B and Hb. Approval was provided by the UBC Biosafety Committee, Certificate number: B13-0096 v TABLE OF CONTENTS ABSTRACT .................................................................................................................................... M., Fu, Z., Dulloor, P., Yen, T., Barron-Casella, E., Savage, W., Van Eyk, J.

The Y440F/Y444F (YFYF), E354A, M363L and S361A variants were found to be deficient in heme transfer. ii PREFACE ...................................................................................................................................... iii TABLE OF CONTENTS ................................................................................................................. v LIST OF TABLES ....................................................................................................................... viii LIST OF FIGURES ......................................................................................................................... ix LIST OF ABBREVIATIONS ......................................................................................................... xi ACKNOWLEDGEMENTS ......................................................................................................... xiii Chapter 1 Introduction ..................................................................................................................... 1 1.1: Staphylococcus aureus .......................................................................................................... 1 1.1.1: Epidemiology and pathogenesis ..................................................................................... 1 1.1.2: Staphylococcus aureus virulence factors .......................................................................

SHOW COMMENTS

Comments Uwo Thesis Binding

The Latest from vesten.ru ©